The shocking incident was filmed on Tunisair flight TU216 at Istanbul Airport in Turkey before the plane was meant to head to Tunis-Carthage International Airport in Tunisia on April 10.
Footage shows a mass brawl which saw passengers lash out and pull each other's hair on a Tunisair flight just before take-off.
The brawl is believed to have originated from a fight about overhead locker space on the plane, according to Business News.
An airline spokesperson said a row broke out between passengers which led to a delay of five hours, and confirmed that an investigation into the incident is underway.
The airline spokesperson confirmed that cabin crew had to ask for the assistance of security teams to calm down tensions before the plane eventually took off.